Molecular Techniques and Methods
Protein Expression, Purification, and Identification
Copy Right ?2001/ Institute of Molecular Development LLC
Electrophoresis of Proteins
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IEF Gel Electrophoresis
Native Gel Electrophoresis
SDS-PAGE
Urea PAGE
Acetic Acid-Urea PAGE
Two-Dimensional Gel Electrophoresis
Starch Gel Electrophoresis
Coomassie Blue Staining of Protein
Silver Staining of Protein
UV cross-linking of RNA and Protein
RNA Gel Mobility Shift Assay
RNA Footprinting
Mercaptoethanol for Protein Denaturation in SDS-PAGE
Northwestern
Protein Expression
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In vitro Translation
Preparation of Over-Expressed Proteins from E. coli
Protein Expression from Cloned Gene in Bacteriophage Lamda
Protein Expression in Yeast-Introduction
Protein Expression in Yeast-Review
Protein Expression in Yeast-Vectors
Protein Expression in Pichia pastoris-Invitrogen
Pichia pastoris fermentation-Invitrogen
Protein Purification and Quantitation
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Protein Purification
Quantitation of Proteins
Protein Identification
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Preparation of Protein Sample for Edman Sequencing (N-terminal sequencing)
Protein Preparation for LC-MS/MS Analysis
Quick Identification of Unknown Protein by Mass Spectrometer Analysis
Identification of RNA-binding Proteins
Yeast 2-hybrid system - Invitrogen
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